Work

Effects of carbon monoxide on one-carbon enzymes and energetics of Clostridium thermoaceticum

Year: 1986

Type: article

Abstract: Clostridium thermoaceticum was cultivated heterotrophically under CO2, carbon monoxide (CO), and H2 gas phases. Formate dehydrogenase (FDH) levels increased 4-fold in CO cultures; formyltetrahydrofolate synthetase (FTS) levels were not influenced by the cultivation gas phases tested. While CO dehydrogenase (CODH) was slightly stimulated in CO cultures, CO-dependent acetyl phosphate-synthesizing system (APSS) activity increased sharply in both CO and H2 cultures. Yglucose values increased, whereas doubling times and acetate to biomass ratios decreased significantly in CO cultures, suggesting that CO cultures were energetically dissimilar to non-CO cultures. This finding, together with the absence of CO-dependent ATP-independent synthesis of formyltetrahydrofolate (formyl-THF), supports the hypothesis that conservation of CO-derived energy involves electron transport phosphorylation.

Source: FEMS Microbiology Letters

DOI: 10.1016/0378-1097(86)90267-3

Authors: Rebecca Kellum, Harold L. Drake

Institution: University of Mississippi

Language: English

Topic: Microbial metabolism and enzyme function

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDGs: Affordable and clean energy, Climate action

Open Access status: bronze

Funder: National Institute of Allergy and Infectious Diseases