Correction: The Natural Pesticide Dihydrorotenone Induces Human Plasma Cell Apoptosis by Triggering Endoplasmic Reticulum Stress and Activating p38 Signaling Pathway
Year: 2020
Type: erratum
Abstract: After publication of this article [1], similarities were noted between the following figure panels:• Fig 1, FACS plots shown for OPM2/5μM DHR and KMS11/5μM DHR • β-actin blots for KMS11 in Fig 3A and for OPM2 in Fig 5A An error was made in preparing Fig 1 such that the OPM2 FACS plot is duplicated in the KMS11 panel.In addition, there are errors in the percentages reported on the U266/0 μM DHR and KMS11/30 μM DHR panels.A corrected Fig 1 is provided with this Correction; dot plots and quantitative data for these FACS analyses are in S1 File.The experiments in Fig 3A and 3C are mislabeled: these experiments were conducted in OPM2 cells, not KMS11 cells, and used the same blots as the GPR78 experiment shown in Fig 5A.Hence, the same β-actin control blot applied for Fig 3A and 5A.The β-actin data shown in the indicated panels were obtained by cutting the blot used for the Bim experiment shown in Fig 3A and then probing the resultant membrane strips (from the same blot) for β-actin, GAPDH, and Bim.The GPR78 experiment was done subsequently by stripping the Bim blot.The available original data for the Fig 3A and 3C experiments are in S1-S3 Files.Both β-actin and GAPDH controls were included in these experiments but only a single loading control was included in each Fig.The control data shown in Fig 3C are mislabeled, these panels show GAPDH results.The LP1/β-actin blot in the original published Fig 3A does not match the original data provided for this experiment and this is addressed in the updated Fig 3.In addition, incorrect percentages are reported in Fig 2C for the right quadrants of the KMS11/DHR+Z-VAD plot; in Fig 4A for DMSO, TMRM + ; and in Fig 4C for the lower left quadrant of the 24 h panel.These errors have been corrected in the updated Figs with this notice.In the new version of Fig 4, the histograms in Fig 4A have been replaced to align with available raw data from the original experiments.Traces, dot plots, and quantitative data supporting these FACS results are in S1 File.The raw data files (.fcs) to support the flow cytometry results are no longer available.All available data to support other results reported in the article are in S1 File.As noted in the article's Materials and Methods, all experiments were repeated (biological replicates, using fresh samples) at least three times.
Source: PLoS ONE
Authors: Jieyu Zhang, Juan Tang, Biyin Cao, Zubin Zhang, Jie Li, Aaron D. Schimmer, Sudan He, Xinliang Mao
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,695
OpenAlex ID: W4246847429