Year: 2017
Type: article
Abstract: Background : Mitochondrial diabetes is primarily caused by β-cell failure, a cell type whose unique properties are important in pathogenesis. Methods : By reducing glucose, we induced energetic stress in two rodent β-cell models to assess effects on cellular function. Results : Culturing rat insulin-secreting INS-1 cells in low glucose conditions caused a rapid reduction in whole cell respiration, associated with elevated mitochondrial reactive oxygen species production, and an altered glucose-stimulated insulin secretion profile. Prolonged exposure to reduced glucose directly impaired mitochondrial function and reduced autophagy. Conclusions : Insulinoma cell lines have a very different bioenergetic profile to many other cell lines and provide a useful model of mechanisms affecting β-cell mitochondrial function.
Source: Wellcome Open Research
Authors: Karl J Morten, Michelle Potter, Luned Badder, Pamela Sivathondan, Rebecca Dragovic, Abigale Neumann, James Gavin, Roshan Shrestha, Svetlana Reilly, Kanchan Phadwal, Tiffany A. Lodge, Angela Borzychowski, Sharon Cookson, Corey Mitchell, Alireza Morovat, Anna Katharina Simon, Johanna Uusimaa, James Hynes, Joanna Poulton
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,128
Funders: Wellcome Trust, Medical Research Council
OpenAlex ID: W4247255634