Correction: Comprehensive Analysis of Leukocytes, Vascularization and Matrix Metalloproteinases in Human Menstrual Xenograft Model
Year: 2011
Type: erratum
Abstract: In our previous study, menstrual-like changes in mouse were provoked through the pharmacologic withdrawal of progesterone with mifepristone following induction of decidualization.However, mouse is not a natural menstruation animal, and the menstruation model using external stimuli may not truly reflect the occurrence and development of the human menstrual process.Therefore, we established a model of menstruation based on human endometrial xenotransplantation.In this model, human endometrial tissues were transplanted subcutaneously into SCID mice that were ovarectomized and supplemented with estrogen and progestogen by silastic implants with a scheme imitating the endocrinological milieu of human menstrual cycle.Morphology, hormone levels, and expression of vimentin and cytokeratin markers were evaluated to confirm the menstrual-like changes in this model.With 28 days of hormone treatment, transplanted human endometrium survived and underwent proliferation, differentiation and disintegration, similar to human endometrium in vivo.Human CD45 + cells showed a peak of increase 28 days post-transplantation. Three days after progesterone withdrawal, mouse CD45 + cells increased rapidly in number and were significantly greater than human CD45 + cell counts.Mouse CD31 + blood vascular-like structures were detected in both transplanted and host tissues.After progesterone withdrawal, the expression levels of matrix metalloproteinases (MMP) 1, 2, and 9 were increased.In summary, we successfully established a human endometrial xenotransplantation model in SCID mice, based on the results of menstrual-like changes in which MMP-1, 2 and 9 are involved.We showed that leukocytes are originated from in situ proliferation in human xenografts and involved in the occurrence of menstruation.This model will help to further understand the occurrence, growth, and differentiation of the endometrium and the underlying mechanisms of menstruation.
Source: PLoS ONE
Authors: Yong Guo, Bin He, Xiangbo Xu, Jiedong Wang
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,382
OpenAlex ID: W4247633397