Faculty Opinions recommendation of Alpha spectrin is essential for morphogenesis and body wall muscle formation in Caenorhabditis elegans.
Year: 2002
Type: peer-review
Abstract: common feature of multicellular animals is the ubiquitous presence of the spectrin cytoskeleton.Although discovered over 30 yr ago, the function of spectrin in nonerythrocytes has remained elusive.We have found that the spc-1 gene encodes the only ␣ spectrin gene in the Caenorhabditis elegans genome.During embryogenesis, ␣ spectrin localizes to the cell membrane in most if not all cells, starting at the first cell stage.Interestingly, this localization is dependent on  spectrin but not  Heavy spectrin.Furthermore, analysis of spc-1 mutants indicates that  spectrin requires ␣ spectrin to be stably recruited to the cell A membrane.Animals lacking functional ␣ spectrin fail to complete embryonic elongation and die just after hatching.These mutant animals have defects in the organization of the hypodermal apical actin cytoskeleton that is required for elongation.In addition, we find that the process of elongation is required for the proper differentiation of the body wall muscle.Specifically, when compared with myofilaments in wild-type animals the myofilaments of the body wall muscle in mutant animals are abnormally oriented relative to the longitudinal axis of the embryo, and the body wall muscle cells do not undergo normal cell shape changes.
Author: Robert K Herman
Institution: University of Minnesota
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4247646459