Synthesis and Applications of Chemical Probes for Human O6-Alkylguanine-DNA Alkyltransferase
Year: 2001
Type: article
Abstract: Novel affinity labels for human O6-alkylguanine-DNA alkyltransferase (hAGT), leading to specific biotinylation of this DNA repair protein (see schematic picture), have been synthesized and characterized. These chemical probes can be used for the specific detection of active hAGT. In addition, a system based on these probes and the display of active hAGT on phage λ has been introduced, which can be applied for the directed molecular evolution of hAGT by using in vitro selection.
Source: ChemBioChem
Authors: Robert Damoiseaux, Antje Keppler, Kai Johnsson
Institution: University of Lausanne
Language: English
Topic: DNA Repair Mechanisms
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W1964774642