HTAPP_CST- Nuclei isolation from frozen tissue v1
Year: 2019
Type: preprint
Abstract: This protocol describes a robust and easy method for nuclei isolation for frozen tissues based on unpublished work by Drokhlyansky et al. This protocol is compatible with 10X droplet-based single-cell technology. This method is part of a toolbox for processing frozen tissue samples, including the CST (this protocol), TST and NST protocols (all available in protocols.io). We recommend users to test all three protocols on their tissue type of interest, and perform side-by-side comparison of the data generated. For the HTAPP project, this protocol was tested and validated for pediatric glioma frozen samples. This protocol was also tested on metastatic breast cancer, ovarian cancer, pediatric sarcoma, and pediatric neuroblastoma frozen samples. For these cancer types, however, TST was the protocol of choice.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4248202290