Year: 1989
Type: article
Abstract: A microorganism producing transglutaminase was screened as an indication of hydroxamate- forming activity. The microbial transglutaminase was purified from the culture filtrate of the strain, S- 8112, which was supposed to belong to the genus Streptoverticillium. The molecular weight of the purified enzyme was found to be about 40,000 on SDS-polyacrylamide gel electrophoresis, the isoelectric point 8.9 and the optimal pH of the reaction 6~7. The present enzyme requires no calcium ions for its activity. Thus, it clearly differs from known transglutaminases derived from mammalian organs, which have been defined as calcium-dependent enzymes.
Authors: Hiroyasu Ando, Masae Adachi, Koichi Umeda, Akira Matsuura, Masahiko Nonaka, Ryosuke Uchio, Haruo Tanaka, Masao Motoki
Institution: Ajinomoto (Japan)
Language: English
Subfield: Biotechnology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W4248559484