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Efforts to transform Heterosigma akashiwo using an Agrobacterium-mediatedapproach v1

Year: 2017

Type: preprint

Abstract: This protocol was developed for transformation of Heterosigma akashiwo byAgrobacterium tumefaciens.H. akashiwodoes not grow on solid medium, so transformants are grown "in bulk" and should not be considered clonal. We have had limited success with this protocol and mayrequire further optimization.

DOI: 10.17504/protocols.io.jsxcnfn

Authors: Vinay K Nagarajan, Monica Accerbi, Pamela J Green

Language: English

Topic: Plant tissue culture and regeneration

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: green

OpenAlex ID: W4251088574

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