Work

Specific Saccharomyces cerevisiae Genes Are Expressed in Response to DNA-Damaging Agents

Year: 1985

Type: article

Abstract: When exposed to DNA-damaging agents, the yeast Saccharomyces cerevisiae induces the expression of at least six specific genes. We have previously identified one damage inducible (DIN) gene as a gene fusion (din-lacZ fusion) whose expression increases in response to DNA-damaging treatments. We describe here the identification of five additional DIN genes as din-lacZ fusions and the responses of all six DIN genes to DNA-damaging agents. Northern blot analyses of the transcripts of two of the DIN genes show that their levels increase after exposure to DNA-damaging agents. Five of the din-lacZ fusions are induced in S. cerevisiae cells exposed to UV light, gamma rays, methotrexate, or alkylating agents. One of the din-lacZ fusions is induced by either UV or methotrexate but not by the other agents. This finding suggests that there are sets of DIN genes that are regulated differently.

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.5.1.75

Authors: S W Ruby, J W Szostak

Institutions: California Institute of Technology, Harvard University, Massachusetts General Hospital, Dana-Farber Cancer Institute

Language: English

Topic: DNA Repair Mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Science Foundation, National Institutes of Health, National Cancer Institute, National Institute of General Medical Sciences

Awards: Regul. of Gene Expression in Prokaryotes and Eukaryotes, Genetic Regulation of Complex Systems in Yeast, Tumor Cell Biology Training Program, G4678295040, G8080906314