Faculty Opinions recommendation of Identification of dynamically regulated microRNA and mRNA networks in developing oligodendrocytes.
Year: 2009
Type: peer-review
Abstract: MicroRNAs (miRNAs) play important roles in modulating gene expression at the posttranscriptional level. In postnatal oligodendrocyte lineage cells, the miRNA expression profile ("microRNAome") contains 43 miRNAs whose expression dynamically changes during the transition from A2B5ϩ oligodendrocyte progenitor cells to premyelinating GalC ϩ cells.The combination of microRNAome profiling with analyses of the oligodendrocyte transcriptome reveals a target bias for a class of miRNAs which includes miR-9.We show that miR-9 is downregulated during oligodendrocyte differentiation.In addition, miR-9 expression level inversely correlates with the expression of its predicted targets, among which is the peripheral myelin protein PMP22.We found that PMP22 mRNA but not protein is detectable in oligodendrocytes, whereas Schwann cells producing PMP22 protein lack miR-9.We demonstrate that miR-9 interacts with the 3Ј untranslated region of PMP22 and downregulates its expression.Our results support models in which miRNAs can act as guardians of the transcriptome.
Author: Sean Ryder
Institution: University of Massachusetts Chan Medical School
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4252453285