Work

Characterization of the genes coding for the F1F0 subunits of the sodium dependent ATPase of Propionigenium modestum

Year: 1992

Type: article

Abstract: The DNA coding for the eight structural genes and uncI of the sodium dependent ATPase of Propionigenium modestum has been cloned and sequenced. Based on sequence homology, the genes were determined to appear in the order uncBEFHAGDC as in several other bacterial species. Minicell experiments revealed that plasmids containing the P. modestum DNA expressed those ATPase polypeptides in Escherichia coli. These were very similar in molecular mass to those obtained from the purified ATPase of P. modestum. No membrane-bound ATPase activity was observed in E. coli unc deletion strains containing the P. modestum ATPase genes. Amino acid alignments which were done with the Fo subunits revealed only a few conservative changes in the highly conserved regions of the polypeptides.

Source: FEMS Microbiology Letters

DOI: 10.1016/0378-1097(92)90559-7

Authors: Lee R. Krumholz, Ursula Esser, Robert D. Simoni

Institution: Stanford University

Language: English

Topic: ATP Synthase and ATPases Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: G1212972069, CLONING AND CHARACTERIZATION OF THE ATPASE OF VIBRIO SP, G8175614836