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Biochemical and Immunological Characterization of Mutant L-M Cells with Altered Levels of Dibutyryl Cyclic AMP-Inducible Alkaline Phosphatase

Year: 1985

Type: article

Abstract: Phosphotyrosine-Sepharose 4B was synthesized and used to purify L-cell alkaline phosphatase. Antibodies to this enzyme interacted with the alkaline phosphatase of strains A-1-2 and A-3-3, mutants that express the enzyme constitutively. This and thermal stability studies suggest that these mutants contain the same alkaline phosphatase isozyme as their parent strain.

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.5.5.1184

Authors: J R Gum, C R Raetz

Language: English

Topic: Alkaline Phosphatase Research Studies

Subfield: Endocrinology, Diabetes and Metabolism

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: G1444002193, BIOGENESIS OF MEMBRANE LIPIDS IN ANIMAL CELLS

OpenAlex ID: W1815805713

FWCI: 0.331

Cites: 25

Cited by: 6

Related to: 10