Tim-3 expression on PD-1+ HCV-specific human CTLs is associated with viral persistence, and its blockade restores hepatocyte-directed in vitro cytotoxicity
Year: 2011
Type: article
Abstract: In the section of Methods titled "Antibodies and flow cytometric analysis," the antibody clone name for the anti-Tim-3 antibody was given incorrectly. The correct sentences appear below: Directly conjugated antibodies against the following surface molecules were used: CCR7-PE-Cy7 (clone 3D12), CD27-APC-H7 (clone M-T271), CD45RA-APC (clone HI100), CD69-FITC (clone L78), HLA-DR-PerCP (clone L243), CD45RO-PE-Cy7 (clone UCHL1), CD3-Pacific Blue (clone UCHT1), CD4-V500 (clone RPA-T4), CD8-Alexa Fluor 700 or CD8-PerCP (clone SK1), and PD-1-FITC (clone MIH4), all from BD Biosciences. The PE-conjugated antibody and the blocking antibody for Tim-3 (clone 344823) were obtained from R&D; the blocking antibody gave results that were comparable to those of 1G5 anti-Tim-3 antibody, provided by
DOI: 10.1172/jci46311
Institutions: Brigham and Women's Hospital, Harvard University
Language: English
Topic: Hepatitis C virus research
Subfield: Hepatology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W4254653468