Work

Dinuclear Ruthenium(II) Complexes as Two‐Photon, Time‐Resolved Emission Microscopy Probes for Cellular DNA

Year: 2014

Type: article

Abstract: The first transition‐metal complex‐based two‐photon absorbing luminescence lifetime probes for cellular DNA are presented. This allows cell imaging of DNA free from endogenous fluorophores and potentially facilitates deep tissue imaging. In this initial study, ruthenium(II) luminophores are used as phosphorescent lifetime imaging microscopy (PLIM) probes for nuclear DNA in both live and fixed cells. The DNA‐bound probes display characteristic emission lifetimes of more than 160 ns, while shorter‐lived cytoplasmic emission is also observed. These timescales are orders of magnitude longer than conventional FLIM, leading to previously unattainable levels of sensitivity, and autofluorescence‐free imaging.

Source: Angewandte Chemie

DOI: 10.1002/ange.201309427

Authors: Elizabeth Baggaley, Martin R. Gill, Nicola H. Green, David Turton, Igor V. Sazanovich, Stanley W. Botchway, Carl Smythe, John W. Haycock, Julia A. Weinstein, Jim A. Thomas

Institutions: University of Sheffield, Rutherford Appleton Laboratory, Research Complex at Harwell, Central Laser Facility

Language: English

Topic: Molecular Sensors and Ion Detection

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: bronze

Funders: Wellcome Trust, Laserlab-Europe, Medical Research Council, Biotechnology and Biological Sciences Research Council, Science and Technology Facilities Council

OpenAlex ID: W4255297055

FWCI: 1.71

Cites: 32

Cited by: 30

Related to: 10