Rapid non-linear image scanning microscopy (Conference Presentation)
Year: 2020
Type: conference-paper
Abstract: Recently, we have developed Image Scanning Microscopy (ISM) that doubles the resolution of a conventional confocal microscope by replacing the confocal pinhole with an imaging detector. Here, we describe theory and realization of a new fully optical non-linear ISM suitable for two-photon excited fluorescence and second-harmonic generation. It provides excellent sensitivity and high frame-rate in combination with two-times improved lateral resolution compared to a conventional two-photon laser-scanning microscope. We demonstrate the performance using fixed and living specimen, as well as hydrogels. The modular design allows straight-forward implementation into existing microscopes. We also present a cost-efficient FLIM-ISM detector providing super-resolved fluorescence lifetime images using two-photon excitation and can be implemented into any confocal microscope.
DOI: 10.1117/12.2541142
Authors: Ingo Gregor, Jörg Enderlein, Robert Ros
Institutions: University of Göttingen, Arizona State University
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4255561127