Work

Caution in Using Proteins Extracted from Exhaled Breath Condensate of Intubated Patients for Pediatric ARDS Studies

Year: 2020

Type: preprint

Abstract: Objective We sought to analyze the quality of proteins extracted from the humidified moisture exchange filter (HMEF) of pediatric acute respiratory distress syndrome (PARDS) patients undergoing invasive mechanical ventilation.Results Proteins were extracted from ten filters from one control and six PARDS subjects. Between 0.69-1.69 mg of protein was extracted from each. Silver stain of these extracts identified only one discrete band compared to many discrete bands in mouse BALF. Liquid chromatography- mass spectrometry of this band and a corresponding band in mouse bronchoalveolar lavage fluid identified them as human and mouse albumin respectively. Multiple other non-degraded proteins were obvious in mouse bronchoalveolar lavage fluid but not proteins extracted from HMEFs.

DOI: 10.21203/rs.3.rs-64380/v1

Authors: Mohit Ojha, Rashika Joshi, Brian Michael Varisco

Institutions: Cincinnati Children's Hospital Medical Center, Cornell University, Weill Cornell Medicine

Language: English

Topic: Respiratory Support and Mechanisms

Subfield: Pulmonary and Respiratory Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Cincinnati Children's Hospital Medical Center, National Heart, Lung, and Blood Institute

Awards: Chymotrypsin-like Elastase 1 in Lung Development and Disease, Cela1 Mediates Stretch-regulated Elastin Remodeling During Alveolar Septation