Work

Pulmonary surfactant proteins A and D are potent endogenous inhibitors of lipid peroxidation and oxidative cellular injury.

Year: 2002

Type: article

Abstract: The purification procedure for all surfactant proteins used in this study included affinity adsorption to carbohydrate columns, elution with EDTA, and dialysis to remove EDTA. We recently discovered that EDTA exhibits atypical dialysis behavior, and despite extensive dialysis, our final SP-A and SP-D preparations contained EDTA. Our conclusion that SP-A and SP-D inhibit surfactant lipid peroxidation at protein concentrations that are physiologically plausible has not changed, but the inhibitory potency of the proteins is less than we originally reported. We intend to submit a methods article to another journal that outlines a scheme for purification of EDTA-free SP-A and SP-D.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(19)33157-6

Authors: James P. Bridges, Harold W. Davis, Mamatha Damodarasamy, Yoshio Kuroki, Gabriel Howles, David Y. Hui, Francis X. McCormack

Language: English

Topic: Antioxidant Activity and Oxidative Stress

Subfield: Biochemistry

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W4256449988

FWCI:

Cites:

Cited by: 4

Related to: 10