Work

Faculty Opinions recommendation of Ribonucleotides are signals for mismatch repair of leading-strand replication errors.

Year: 2013

Type: peer-review

Abstract: To maintain genome stability, mismatch repair of nuclear DNA replication errors must be directed to the nascent strand, likely by DNA ends and PCNA.Here we show that the efficiency of mismatch repair in Saccharomyces cerevisiae is reduced by inactivating RNase H2, which nicks DNA containing ribonucleotides incorporated during replication.In strains encoding mutator polymerases, this reduction is preferential for repair of mismatches made by leading strand DNA polymerase ε as compared to lagging strand DNA polymerase δ.The results suggest that RNase H2-dependent processing of ribonucleotides transiently present in DNA after replication may direct mismatch repair to the continuously replicated nascent leading strand.

Source: Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature

DOI: 10.3410/f.718001967.793476723

Author: Michael Lichten

Institutions: National Institutes of Health, National Cancer Institute, Center for Cancer Research

Language: English

Topic: DNA Repair Mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of Environmental Health Sciences, NHLBI Division of Intramural Research

Awards: Structure-Function Studies Of DNA Replication Fidelity, G5792972300

OpenAlex ID: W4256572685

Cites: 30

Cited by:

Related to: 10