FGFR3 is expressed by human primordial germ cells and is repressed after meiotic initiation to form primordial oocytes
Year: 2022
Type: article
Abstract: Human germ cell development is a highly regulated process beginning soon after embryo implantation with the specification of primordial germ cells (PGCs) and ending in adulthood with the differentiation of gametes. Here, we show that fibroblast growth factor receptor 3 (FGFR3) is expressed by human PGCs during the first and second trimester, becoming repressed as PGCs differentiate into primordial oocytes. Using fluorescence-activated cell sorting (FACS) with antibodies that recognize FGFR3 followed by single-cell RNA sequencing, we show that isolating FGFR3-positive cells enriches for human PGCs. Taken together, FGFR3 could be used in future studies as a strategy to identify maturing PGCs in vitro.
Source: Stem Cell Reports
Language: English
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,800
Funders: Howard Hughes Medical Institute, Boehringer Ingelheim, National Institutes of Health, University of California, Los Angeles, National Cancer Institute, National Institute of General Medical Sciences, Jonsson Comprehensive Cancer Center, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California Los Angeles, Eunice Kennedy Shriver National Institute of Child Health and Human Development, David Geffen School of Medicine, University of California, Los Angeles
OpenAlex ID: W4280558175