Work

Simultaneous recording of breathing and neural activity in awake behaving mice

Year: 2022

Type: article

Abstract: This protocol provides a pipeline for simultaneous recording of breathing and neural activities in awake, behaving mice. Breathing is recorded with thermistor probes implanted in the nasal cavity, which can be easily integrated with neural activity monitoring approaches such as fiber photometry. Here, we detail the procedures of the thermistor probe assembly, surgery, recording system setup, and data analysis. This protocol can be applied to investigate respiratory physiology and breathing changes during natural behaviors. For complete details on the use and execution of this protocol, please refer to Liu et al. (2022).

Source: STAR Protocols

DOI: 10.1016/j.xpro.2022.101412

Authors: Shijia Liu, Sung Han

Institutions: Salk Institute for Biological Studies, University of California San Diego

Language: English

Topic: Neuroscience of respiration and sleep

Subfield: Endocrine and Autonomic Systems

Field: Neuroscience

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,500

Funders: Brain Research Foundation, National Institutes of Health, National Institute of Mental Health