Intraoperative administration of isoflurane improves survival in rats exposed to caecal ligation and puncture
Year: 2022
Type: article
Abstract: Background Emerging data suggest that volatile anaesthetic agents may be protective during critical illness. Methods Three-month-old Sprague Dawley rats were randomly allocated to one of four groups: isoflurane during surgery followed by 3 days of isoflurane 0.8% (and intralipid i.v.), propofol during surgery and 314 μg kg −1 h −1 propofol for 3 days, isoflurane during surgery and intralipid for 3 days, and propofol during surgery and intralipid for 3 days. After induction with propofol or isoflurane, rats breathed oxygen 100% spontaneously via a nose cone. Propofol or intralipid was administered through a 22-gauge jugular vein i.v. catheter. Caecal ligation and puncture was performed through a paramedian incision. The surgical concentration of isoflurane was kept at 2%, and propofol was maintained at 800 μg kg −1 h −1 . After recovery and 3 days of exposure to intralipid or anaesthetic agents, the rats were allowed to roam free in an adequately vented, temperature- and humidity-controlled cage with food and water ad libitum . Results Rats that received isoflurane for 3 days survived longer than the postoperative propofol group ( P =0.0002, log-rank test). Among rats receiving no postoperative anaesthetic, those receiving isoflurane during surgery survived longer than those that received propofol during surgery group ( P =0.0081). Conclusions Exposure to isoflurane, as opposed to propofol, may improve survival in rats exposed to caecal ligation and puncture.
Source: BJA Open
Authors: Keita Ikeda, Hari P. Osuru, Robert H. Thiele
Institution: University of Virginia Health System
Language: English
Subfield: Anesthesiology and Pain Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,912
OpenAlex ID: W4281261887