Work

LGG-50. Human induced pluripotent stem cell engineering establishes a humanized mouse platform for pediatric low-grade glioma modeling

Year: 2022

Type: conference-abstract

Abstract: A major obstacle to identifying improved treatments for pediatric low-grade brain tumors (gliomas) is the inability to reproducibly generate human xenografts. To surmount this barrier, we leveraged human induced pluripotent stem cell (hiPSC) engineering to generate low-grade glioma (LGG) lesions representing the two most common pediatric pilocytic astrocytoma-associated molecular alterations, NF1 loss and KIAA1549:BRAF fusion. Using hiPSCs, we identified the susceptible cells of origin for these tumors, and demonstrated that the resulting tumors retain LGG histologic features for at least 6 months in vivo. Finally, this platform enabled the successful long-term growth of patient-derived pLGGs in vivo. Taken together, these avatars establish tractable experimental humanized platforms to elucidate the pathogenesis of childhood brain tumors.

Source: Neuro-Oncology

DOI: 10.1093/neuonc/noac079.362