A lymphatic route for a hyperbranched heteroglycan from Radix Astragali to trigger immune responses after oral dosing
Year: 2022
Type: article
Abstract: Gut barrier makes a huge research gap between in vivo and in vitro studies of orally bioactive polysaccharides: whether/how they contact the related cells in vivo. A hyperbranched heteroglycan RAP from Radix Astragali, exerting antitumor and immunomodulatory effects in vitro and in vivo, is right an example. Here, we determined first that RAP's antitumor activity is immune-dependent. Being undegraded and non-absorbing, RAP quickly entered Peyer's patches (PPs) in 1 h where it directly targeted follicle dendritic cells and initiated antitumor immune responses. RAP was further delivered to mesenteric lymph node, bone marrow, and tumor. By contrast, the control Dendrobium officinale polysaccharide did not enter PPs. These findings revealed a blood/microbiota-independent and selective lymphatic route for orally administrated RAP to directly contact immune cells and trigger antitumor immune responses. This route bridges the research gap between the in vitro and in vivo studies and might apply to many other bioactive polysaccharides.
Source: Carbohydrate Polymers
Authors: Quanwei Zhang, Lifeng Li, Shuang Hao, Man Liu, Chuying Huo, Jianjun Wu, Hongbing Liu, Wanrong Bao, Hongming Zheng, Zhipeng Li, Huiyuan Cheng, Hauyee Fung, Tinlong Wong, Pingchung Leung, Shunchun Wang, Ting Li, Ge Zhang, Min Li, Zhongzhen Zhao, Wei Jia, Zhaoxiang Bian, Timothy Mitchison, Jingchao Zhang, Aiping Lyu, Quanbin Han, Handong Sun
Institutions: Hong Kong Baptist University, Zhengzhou University, First Affiliated Hospital of Zhengzhou University, Chinese University of Hong Kong, Shanghai University of Traditional Chinese Medicine, Macau University of Science and Technology, Harvard University, Kunming Institute of Botany, Chinese Academy of Sciences
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,680
OpenAlex ID: W4281730836