Work

Correction: MDM2 E3 ligase activity is essential for p53 regulation and cell cycle integrity

Year: 2022

Type: erratum

Abstract: Fig 3. p53-null Mdm2 la/la MEFs and sarcoma cells have defects in and increased and G2-M transition hyperploidy.(A) Cell cycle profiles of Mdm2 la/la : TP53 R/R and Mdm2 +/+ : TP53 R/R MEFs (passage 6) by flow cytometry.Dip, diploid, An, aneuploid.(B) Increased phospho-Histone 3 at Serine 10 (pH3(S10) in p53-deficient Mdm2 la/la sarcoma tissues.Representative histochemical staining of pH3(S10) (a, b) and Ki67 (c, d) in sarcoma tissues from p53 -/-: Mdm2 +/+ (a, c) or p53 -/-: Mdm2 la/la (b, d) mice.Left images at 10x magnification and at 40x magnification of image areas in frame shown on the right.(C) Quantitative analysis of pH3(S10) staining in two p53 -/-: Mdm2 +/+ and three p53 -/-: Mdm2 la/la sarcoma samples.� , t test, p = 0.0106.(D) Quantitative analysis of Ki67-positive cells in two p53 -/-: Mdm2 +/+ and three p53 -/-: Mdm2 la/la sarcoma samples.ns, t test, p = 0.604.(E) Mdm2 +/+ -tetp53 and Mdm2 la/la -tetp53 MEFs were used for BrdU labeling experiments.Gating settings are shown to define viable, singlet and BrdU-positive cells.(F) Diploid S (Dip S) and hyperploid S (Hyp S) fractions of Mdm2 +/+ -tetp53 and Mdm2 la/la -tetp53 MEFs were presented.(G)

Source: PLoS Genetics

DOI: 10.1371/journal.pgen.1010293