Work

Protocol for assessing ex vivo lipolysis of murine adipose tissue

Year: 2022

Type: article

Abstract: Here, we provide a detailed protocol for assessing ex vivo lipolysis of subcutaneous and visceral white adipose tissue. We describe a robust approach to detect depot-specific changes in lipolytic potential under basal and beta-adrenergic receptor-stimulated conditions. Given that adipose tissue plays a critical role in systemic metabolic health, this experimental protocol can be used to determine changes in adipose tissue function in health and disease.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2022.101518

Authors: Debasish Roy, Julia M. Myers, Andrea Tedeschi

Institutions: The Ohio State University Wexner Medical Center, The Ohio State University

Language: English

Topic: Adipose Tissue and Metabolism

Subfield: Physiology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,500