Work

Reversal of obesity development in Ceacam1 −/− male mice by bone marrow transplantation or introduction of the human CEACAM1 gene

Year: 2022

Type: article

Abstract: Objective Although Ceacam1−/− male mice become obese on normal chow, the effect of bone marrow transplantation or introduction of the carcinoembryonic antigen‐related cell adhesion molecule 1 (CEACAM1) gene has not been studied, to the knowledge of the authors. Methods This study analyzed Ceacam1−/− mice on normal diet or high‐fat diet (HFD), including effects of bone marrow transplantation or introduction of the CEACAM1 gene. Results Male Ceacam1−/− mice on normal diet versus HFD for 24 weeks gained significantly more weight than controls, and Ceacam1−/− mice aged up to 2 years had a high frequency of liver cancer. Transplantation of wild‐type bone marrow into Ceacam1−/− mice or introduction of the human CEACAM1 gene fully or partially reversed the obesity phenotype. Liver lipidomics on Ceacam1−/− versus wild‐type controls on an HFD revealed a significant increase in diacyl glycerides. An increase in fatty acid transporter CD36 levels further suggests that loss of Ceacam1 leads to a major dysregulation of free fatty acid uptake. Conclusions CEACAM1 expression in both the liver and immune cells regulates obesity and lipid storage pathways in the liver. Bone marrow reconstitution of the immune system or introduction of the human CEACAM1 gene can fully or partially reverse the phenotype.

Source: Obesity

DOI: 10.1002/oby.23457

Authors: Zhifang Zhang, Deirdre La Placa, Gabriel Gugiu, Alyssa Thunen, Keith Le, John E. Shively

Institutions: City Of Hope National Medical Center, City of Hope

Language: English

Topic: Adipokines, Inflammation, and Metabolic Diseases

Subfield: Epidemiology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,600