Year: 2022
Type: preprint
Abstract: Summary In the mitochondrial genome of kinetoplastids, the targeted insertion and deletion of uridine in cryptic mRNA is essential to generate functional protein-coding mRNAs, but such process leads to a highly hetergenous and complex RNA population. Few tools were developped to perform alignment based on a T depleted strategy for such mRNA population, however to our knowledge no downstream analysis tool is available so far to allow easy data processing and interpretation. Here, we fill this gap with the T-less-Alignment-Toolkit , which provides useful functions such as duplication removal with UMI, alignment strandness analysis, statistical summary and visualization. Availability and Implementation Scripts and toolkits can be downloaded at https://gitee.com/Zhanglab/tless-alignment-toolkit
Institutions: ShanghaiTech University, Boston University, Boston Medical Center
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4284890813