Protocol for intracellular recording from mouse intrinsic cardiac neurons v1
Year: 2022
Type: preprint
Abstract: Basic protocol for isolation and intracellular recording from mouse intrinsic cardiac neurons is presented. The intrinsic cardiac ganglia, containing hundreds of intrinsic cardiac neurons (ICNs), lie on the dorsal epicardial surface of the mouse heart. These cells receive innervation, principally, from preganglionic parasympathetic neurons of the brainstem by axonal projections within the vagus nerve. These neurons project to multiple targets within the heart (e.g. nodal cells, vascular smooth muscle, myocardium) to affect cardiac dynamics (e.g. heart rate, contractility). This protocol was used for assessing the membrane properties of ICNs sampled from from both control and diabetic mice as presented in the published manuscript (PMID: 31625779) and accompanying dataset.
Author: John D Tompkins
Language: English
Subfield: Cardiology and Cardiovascular Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4285009341