Year: 2022
Type: article
Abstract: Nanoscale optical resolution with a large field of view is a critical feature for many research and industry areas, such as semiconductor fabrication, biomedical imaging, and nanoscale material identification. Several scanning microscopes have been developed to resolve the inverse relationship between the resolution and field of view; however, those scanning microscopes still rely upon fluorescence labeling and complex optical systems. To overcome these limitations, we developed a dual-camera acoustofluidic nanoscope with a seamless image merging algorithm (alpha-blending process). This design allows us to precisely image both the sample and the microspheres simultaneously and accurately track the particle path and location. Therefore, the number of images required to capture the entire field of view (200 × 200 μm) by using our acoustofluidic scanning nanoscope is reduced by 55-fold compared with previous designs. Moreover, the image quality is also greatly improved by applying an alpha-blending imaging technique, which is critical for accurately depicting and identifying nanoscale objects or processes. This dual-camera acoustofluidic nanoscope paves the way for enhanced nanoimaging with high resolution and a large field of view.
Source: Microsystems & Nanoengineering
Institutions: Duke University, University of Dayton
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,790
Funders: National Science Foundation, National Institutes of Health, National Institute on Aging, National Heart, Lung, and Blood Institute, National Institute of General Medical Sciences, Division of Civil, Mechanical and Manufacturing Innovation, National Center for Advancing Translational Sciences, Eunice Kennedy Shriver National Institute of Child Health and Human Development
Awards: G1034614284, Acoustofluidic Separation (AFS), Purification and Raman Spectral Fingerprinting of Single EVs: From Cell of Origin to Target Cell and Biofluids, Collaborative Research: Acoustic Holography Enabled Additive Manufacturing of High-resolution Multifunctional Composites, AFS/SERS Saliva-based SARS-CoV-2 Earliest Infection and Antibodies Detection, Development of a digital acoustofluidic system for automating liquid handling in biomedical research, G5971577425, Three-Dimensional (3D) Acoustofluidic Scanning Nanoscope with Super Resolution and Large Field of View, Enabling Efficient, Fast, Biocompatible Exosome Separation via Acoustofluidics, An acoustofluidic device for diagnosing preclinical Alzheimer's disease, Integrated Acoustofluidic Plasmonic Molecular Diagnostic System for Detecting MicroRNA Biomarkers, Graduate Research Fellowship Program (GRFP), An Acoustofluidic Device for Biocompatible Platelet Separation, Acoustofluidic Separation of Placental Nanovesicle Subpopulations in Obstetrical Diseases
OpenAlex ID: W4285083455