Cerebral Aβ deposition in an Aβ-precursor protein-transgenic rhesus monkey
Year: 2022
Type: article
Abstract: With the ultimate goal of developing a more representative animal model of Alzheimer's disease (AD), two female amyloid-β-(Aβ) precursor protein-transgenic (APPtg) rhesus monkeys were generated by lentiviral transduction of the APP gene into rhesus oocytes, followed by in vitro fertilization and embryo transfer. The APP-transgene included the AD-associated Swedish K670N/M671L and Indiana V717F mutations (APPSWE/IND) regulated by the human polyubiquitin-C promoter. Overexpression of APP was confirmed in lymphocytes and brain tissue. Upon sacrifice at 10 years of age, one of the monkeys had developed Aβ plaques and cerebral Aβ-amyloid angiopathy in the occipital, parietal, and caudal temporal neocortices. The induction of Aβ deposition more than a decade prior to its usual emergence in the rhesus monkey supports the feasibility of creating a transgenic nonhuman primate model for mechanistic analyses and preclinical testing of treatments for Alzheimer's disease and cerebrovascular amyloidosis.
Source: Aging Brain
Authors: Anthony W.S. Chan, In Ki Cho, Chun-Xia Li, Xiaodong Zhang, Sudeep Patel, Rebecca Rusnak, Jessica Raper, Jocelyne Bachevalier, Sean P. Moran, Tim Chi, Katherine H. Cannon, Carissa E. Hunter, Ryan C. Martin, Hailian Xiao, Shang-Hsun Yang, Sanjeev Gumber, James G. Herndon, Rebecca F. Rosen, William T. Hu, James J. Lah, Allan I. Levey, Yoland Smith, Lary C. Walker
Institutions: Emory University, Emory National Primate Research Center
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,000
OpenAlex ID: W4285260795