Work

Recent Advances in Improving Gene-Editing Specificity through CRISPR–Cas9 Nuclease Engineering

Year: 2022

Type: review

Abstract: CRISPR-Cas9 is the state-of-the-art programmable genome-editing tool widely used in many areas. For safe therapeutic applications in clinical medicine, its off-target effect must be dramatically minimized. In recent years, extensive studies have been conducted to improve the gene-editing specificity of the most popular CRISPR-Cas9 nucleases using different strategies. In this review, we summarize and discuss these strategies and achievements, with a major focus on improving the gene-editing specificity through Cas9 protein engineering.

Source: Cells

DOI: 10.3390/cells11142186

Authors: Xiaoqiang Huang, Dongshan Yang, Jifeng Zhang, Jie Xu, Y. Eugene Chen

Institutions: University of Michigan, Michigan Medicine

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,303

Funders: Cystic Fibrosis Foundation, National Institutes of Health, National Heart, Lung, and Blood Institute, National Institute of General Medical Sciences