Distinctive Energy Profile of Water-Soluble, Thiolate-Protected Gold Nanoparticles as Potential Molecular Marker for Vulnerable Plaque Detection with XFCT Imaging.
Year: 2020
Type: article
Abstract: Purpose: X-ray CT plays a pivotal role in diagnostic imaging, radiotherapy, and its indispensable contribution to preclinical small animal imaging research. This study characterizes a distinctive energy spectrum of a novel 3-mercaptobenzoic-acid (3MBA)-protected-144-atoms gold-nanoparticles (3MBA-Au-144-NPs) after X-ray excitation and detects vulnerable atherosclerotic plaques non-invasively using this novel contrast agent in mice carotid arteries for the first time to the best of our knowledge. Methods: detection of unstable plaques, we developed atherosclerotic mice model after feeding them 1% high cholesterol diet (HCD) for four weeks before diabetic was induced by intraperitoneal injection of streptozotocin (STZ) to accelerate the plaque progression. Two weeks after the diabetic induction, surgically left carotid artery was ligated. Two weeks after the surgical ligation, a 250 μL of 3MBA-Au-144-NPs was IV injected after 6 hours of fasting. One hour after injection, the mice were imaged non-invasively with a cone-beam micro-CT system. Results: =0.999) with respect to different concentrations of 3MBA-Au-144-NPs. High XRF signal was detected in the left carotid artery at 2 mm below the ligation and in aortic arch. Non-ligated right carotid artery (negative control) showed no such signal. Conclusion: XFCT imaging.
Source: PubMed
Authors: Raiyan T Zaman, Don Vernekhol, Lei Xing
Institutions: Harvard University, Stanford Medicine, Stanford University
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W4285711342