Work

Characterization of N- and C-terminal endogenously tagged Tyrosyl-DNA phosphodiesterase 2 (TDPT-1) C. elegans strains

Year: 2022

Type: article

Abstract: Tyrosyl-DNA phosphodiesterase 2 (TDP2) is a multifunctional protein involved in signal transduction, transcription regulation, and DNA repair (Esguerra et al., 2007; Pei et al., 2003; Pommier et al., 2016; Pype et al., 2000). In its role as a DNA repair enzyme, the phosphodiesterase activity of TDP2 is involved in the removal of trapped Top-2-DNA protein complexes (TOP-2-DPCs) (Nitiss, 2009; Schellenberg et al., 2016; Shi et al., 2012). Unrepaired TOP2-DPCs block the progression of RNA and DNA polymerases. This can lead to accumulation of recombinogenic intermediates that can catalyze the generation of deletions and translocations. TOP2-DPCs can therefore lead to mutagenesis, neurological diseases, and carcinogenesis (Riccio et al., 2020).

Source: PubMed Central

DOI: 10.17912/micropub.biology.000540

Authors: Nirajan Bhandari, Shannon C. Pfeiffer, Aimee Jaramillo-Lambert

Institution: University of Delaware

Language: English

Topic: DNA Repair Mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institute of General Medical Sciences, Eunice Kennedy Shriver National Institute of Child Health and Human Development