Proteomic Profiling of Plasma Biomarkers Associated With Return to Sport Following Concussion: Findings From the NCAA and Department of Defense CARE Consortium
Year: 2022
Type: article
Study design: Observational Study
Abstract: Objective To investigate the plasma proteomic profiling in identifying biomarkers related to return to sport (RTS) following a sport-related concussion (SRC). Methods This multicenter, prospective, case-control study was part of a larger cohort study conducted by the NCAA-DoD Concussion Assessment, Research, and Education (CARE) Consortium, athletes (n = 140) with blood collected within 48 h of injury and reported day to asymptomatic were included in this study, divided into two groups: (1) recovery <14-days (n = 99) and (2) recovery ≥14-days (n = 41). We applied a highly multiplexed proteomic technique that uses DNA aptamers assay to target 1,305 proteins in plasma samples from concussed athletes with <14-days and ≥14-days. Results We identified 87 plasma proteins significantly dysregulated (32 upregulated and 55 downregulated) in concussed athletes with recovery ≥14-days relative to recovery <14-days groups. The significantly dysregulated proteins were uploaded to Ingenuity Pathway Analysis (IPA) software for analysis. Pathway analysis showed that significantly dysregulated proteins were associated with STAT3 pathway, regulation of the epithelial mesenchymal transition by growth factors pathway, and acute phase response signaling. Conclusion Our data showed the feasibility of large-scale plasma proteomic profiling in concussed athletes with a <14-days and ≥ 14-days recovery. These findings provide a possible understanding of the pathophysiological mechanism in neurobiological recovery. Further study is required to determine whether these proteins can aid clinicians in RTS decisions.
Source: Frontiers in Neurology
Authors: Rany Vorn, Sara Mithani, Christina Devoto, Timothy B. Meier, Chen Lai, Sijung Yun, Steven P. Broglio, Thomas W. McAllister, Christopher C. Giza, Hyung-Suk Kim, Daniel Huber, Jaroslaw Harezlak, Kenneth L. Cameron, Gerald McGinty, Jonathan Jackson, Kevin M. Guskiewicz, Jason P. Mihalik, Alison Brooks, Stefan Duma, Steven Rowson, Lindsay D. Nelson, Paul Pasquina, Michael A. McCrea, Jessica M. Gill
Institutions: National Institutes of Health, Johns Hopkins University, National Institute of Nursing Research, The University of Texas at San Antonio Health Science Center, Henry M. Jackson Foundation, Medical College of Wisconsin, Uniformed Services University of the Health Sciences, Center for Neuroscience and Regenerative Medicine, University of Michigan, Indiana University School of Medicine, University of California, Los Angeles, Indiana University Bloomington, Indiana University, Keller Army Community Hospital, United States Air Force Academy, University of North Carolina at Chapel Hill, University of Wisconsin–Madison, Virginia Tech
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,950
OpenAlex ID: W4285794697