Work

Polishing De Novo Nanopore Assemblies of Bacteria and Eukaryotes With FMLRC2

Year: 2023

Type: article

Abstract: As the accuracy and throughput of nanopore sequencing improve, it is increasingly common to perform long-read first de novo genome assemblies followed by polishing with accurate short reads. We briefly introduce FMLRC2, the successor to the original FM-index Long Read Corrector (FMLRC), and illustrate its performance as a fast and accurate de novo assembly polisher for both bacterial and eukaryotic genomes.

Source: Molecular Biology and Evolution

DOI: 10.1093/molbev/msad048

Authors: Q X Charles Mak, Ryan R Wick, James Matthew Holt, Jeremy R Wang

Institutions: National University of Singapore, The University of Melbourne, Pacific Biosciences (United States), University of North Carolina at Chapel Hill

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,255

Funders: National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases