3D diffractive imaging of nanoparticle ensembles using an x-ray laser
Year: 2020
Type: article
Abstract: Single particle imaging at x-ray free electron lasers (XFELs) has the potential to determine the structure and dynamics of single biomolecules at room temperature. Two major hurdles have prevented this potential from being reached, namely, the collection of sufficient high-quality diffraction patterns and robust computational purification to overcome structural heterogeneity. We report the breaking of both of these barriers using gold nanoparticle test samples, recording around 10 million diffraction patterns at the European XFEL and structurally and orientationally sorting the patterns to obtain better than 3-nm-resolution 3D reconstructions for each of four samples. With these new developments, integrating advancements in x-ray sources, fast-framing detectors, efficient sample delivery, and data analysis algorithms, we illuminate the path towards sub-nanometer biomolecular imaging. The methods developed here can also be extended to characterize ensembles that are inherently diverse to obtain their full structural landscape.
Source: Optica
Authors: Kartik Ayyer, P. Lourdu Xavier, Johan Bielecki, Zhou Shen, Benedikt J. Daurer, Amit K. Samanta, Salah Awel, Richard Bean, Anton Barty, Martin Bergemann, Tomas Ekeberg, Armando D. Estillore, Hans Fangohr, Klaus Giewekemeyer, Mark S. Hunter, Mikhail Karnevskiy, Richard A. Kirian, Henry Kirkwood, Yoonhee Kim, Jayanath Koliyadu, Holger Lange, Romain Letrun, Jannik Lübke, Thomas Michelat, Andrew J. Morgan, Nils Roth, Tokushi Sato, Marcin Sikorski, Florian Schulz, John C. H. Spence, Patrik Vagovic, Tamme Wollweber, Lena Worbs, Oleksandr Yefanov, Yulong Zhuang, Filipe R. N. C. Maia, Daniel A. Horke, Jochen Küpper, N. Duane Loh, Adrian P. Mancuso, Henry N. Chapman
Institutions: Universität Hamburg, Deutsches Elektronen-Synchrotron DESY, Center for Free-Electron Laser Science, Max Planck Institute for the Structure and Dynamics of Matter, Hamburg Centre for Ultrafast Imaging, European X-Ray Free-Electron Laser, National University of Singapore, Uppsala University, SLAC National Accelerator Laboratory, Linac Coherent Light Source, Arizona State University, The University of Melbourne, Lawrence Berkeley National Laboratory, National Energy Research Scientific Computing Center, Radboud University Nijmegen, La Trobe University
Language: English
Subfield: Radiation
Field: Physics and Astronomy
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,208
Funders: National Science Foundation, Joachim Herz Stiftung, European Commission, Human Frontier Science Program, Helmholtz-Gemeinschaft, Deutsche Forschungsgemeinschaft, Carl Tryggers Stiftelse för Vetenskaplig Forskning, Vetenskapsrådet, Helmholtz Association, Röntgen-Ångström Cluster, BioXFEL Science and Technology Center, European Research Council, Deutsches Elektronen-Synchrotron
Awards: G103193202, G1230266125, G1307938796, G1803495163, G2109279595, G2306834673, G3207356259, EXC 2056: CUI: Advanced Imaging of Matter, G393177772, G4104599821, G4318061833, G4675369358, G4760961572, G5631081224, G5702163051, EXC 1074: Hamburger Zentrum für ultraschnelle Beobachtung (CUI): Struktur, Dynamik und Kontrolle von Materie auf atomarer Skala, G8433356701, Biology with X-ray Lasers
OpenAlex ID: W3109910286