Work

Characterization of plasma exosomal microRNAs in responding to radiotherapy of human esophageal squamous cell carcinoma

Year: 2022

Type: article

Abstract: Radiotherapy is one of the main treatment methods for esophageal squamous cell carcinoma (ESCC). Previous research has shown that plasma exosomal microRNAs (miRNAs) can predict therapeutic outcome. In the present study, to identify potential exosomal miRNAs that respond to radiotherapy, plasma exosomal miRNAs from ESCC patients undergoing radiotherapy were isolated and sequenced. Upregulated and downregulated miRNAs were detected from patients pre‑ and post‑radiotherapy, and it was found that they play distinct roles in DNA damage process and endosomal mediated transport. Based on wound healing and Cell Counting Kit‑8 assays in TE‑1 human esophageal cancer cells, it was identified that representative miRNA miR‑652 and miR‑30a alter migration but not proliferation. The present findings identified differentially expressed miRNAs in responding to radiotherapy, and added a reference to explore non‑invasive plasma biomarkers to evaluate therapeutic effects in ESCC.

Source: Molecular Medicine Reports

DOI: 10.3892/mmr.2022.12803

Authors: Nan Miao, Wenjie Cai, Sijia Ding, Yajuan Liu, Wanhua Chen, Tao Sun

Institutions: Huaqiao University, Fujian Medical University

Language: English

Topic: Extracellular vesicles in disease

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Funder: Fundamental Research Funds for the Central Universities