EV-associated miRNAs from peritoneal lavage as potential diagnostic biomarkers in colorectal cancer
Year: 2019
Type: article
Study design: Observational Study
Abstract: Colorectal cancer (CRC) is the third leading cause of cancer-related mortality worldwide. Current sys‑ tematic methods for diagnosing have inherent limitations so development of a minimally-invasive diagnosis, based on the identifcation of sensitive biomarkers in liquid biopsies could therefore facilitate screening among population at risk. In this study, we aim to develop a novel approach to identify highly sensitive and specifc biomarkers by investigating the use of extracellular vesicles (EVs) isolated from the peritoneal lavage as a source of potential miRNA diagnostic biomarkers. We isolated EVs by ultracentrifugation from 25 ascitic fuids and 25 peritoneal lavages from non-cancer and CRC patients, respectively. Analysis of the expression of EV-associated miRNAs was performed using Taqman OpenArray technology through which we could detect 371 miRNAs. 210 miRNAs were signifcantly dysregulated (adjusted p value<0.05 and abs(logFC)≥1). The top-10 miR‑ NAs, which had the AUC value higher than 0.95, were miRNA-199b-5p, miRNA-150-5p, miRNA-29c-5p, miRNA-218-5p, miRNA-99a-3p, miRNA-383-5p, miRNA-199a-3p, miRNA-193a-5p, miRNA-10b-5p and miRNA-181c-5p. This finding opens the avenue to the use of EV-associated miRNA of peritoneal lavages as an untapped source of biomarkers for CRC.
Institutions: Universitat Autònoma de Barcelona, Universitat de Lleida, Bellvitge University Hospital, Institut d'Investigació Biomédica de Bellvitge, Instituto de Investigación Biomédica de Lleida, Vall d'Hebron Institut de Recerca, Centro de Investigación Biomédica en Red de Cáncer, Barcelona Institute of Science and Technology, Centre for Genomic Regulation, Hospital Universitari Arnau de Vilanova, CIC bioGUNE, Ikerbasque, Universitat Pompeu Fabra
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4288316089