Expression of SIRT1 signaling pathway in intestinal tissues of neonatal necrotizing enterocolitis
Year: 2019
Type: article
Study design: Observational Study
Abstract: Objective: To analyze the expression of sirtuin type 1 (SIRT1) signal pathway in intestinal \ntissues of neonatal necrotizing enterocolitis (NEC), and to preliminarily explore the role of \nSIRT1 in the occurrence of NEC. Methods: From January 2017 to June 2017, the ileal tissues \nof 35 neonates with NEC underwent one-stage fistula treatment were selected as NEC group, \nand the ileal tissues of these 35 neonates underwent two-stage fistula treatment were selected \nas control group. The expression levels of SIRT1, transcription factor-nuclear factor (NF-κB), \nand SUMO specific protease 1 (SENP1) were detected by qRT-PCR; the expressions of SIRT1, \nNF-κB, and SENP1 were detected by immunohistochemistry and Western blotting (WB). \nResults: SIRT1 mRNA, protein positive expression rate, average optical density, and relative \nprotein expression in intestinal tissues of children in NEC group were significantly lower than \nthose in control group (P<0.05), however, the expression of NF-κB, the SENP1 mRNA and \nprotein positive expression rates, the average optical density, and relative protein expression \nwere significantly increased (P<0.05). Conclusion: SIRT1 is low expressed in intestinal tissues \nof children with NEC, the possible reason is that SIRT1 signaling pathway is suppressed and \nthen NEC occurs.
Authors: Lan Zhang, Bo-Liang Wang, Li Wang, Qiong Gao, Zhao-Xia Xi, Yan-Hong Gui, Hui-Ping Wang
Language: English
Topic: Infant Nutrition and Health
Subfield: Nutrition and Dietetics
Field: Nursing
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W4288378971
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