Elevated c-di-GMP Levels and Expression of the Type III Secretion System Promote Corneal Infection by Pseudomonas aeruginosa
Year: 2022
Type: article
Abstract: Pseudomonas aeruginosa is generally believed to establish biofilm-associated infections under the regulation of the secondary messenger c-di-GMP. To evaluate P. aeruginosa biofilm physiology during ocular infections, comparative transcriptomic analysis was performed on wild-type P. aeruginosa PAO1, a Δ wspF mutant strain (high c-di-GMP levels), and a p lac - yhjH -containing strain (low c-di-GMP levels) from mouse corneal infection, as well as in vitro biofilm and planktonic cultures. The c-di-GMP content in P. aeruginosa during corneal infection was monitored using a fluorescent c-di-GMP reporter strain. Biofilm-related genes were induced in in vivo PAO1 compared to in vitro planktonic bacteria. Several diguanylate cyclases and phosphodiesterases were commonly regulated in in vivo PAO1 and in vitro biofilm compared to in vitro planktonic bacteria.
Source: Infection and Immunity
DOI: 10.1128/iai.00061-22
Authors: Joey Kuok Hoong Yam, Thet Tun Aung, Song Lin Chua, Yingying Cheng, Gurjeet Singh Kohli, Jianuan Zhou, Florentin Constancias, Yang Liu, Zhao Cai, May Margarette Santillan Salido, Daniela I. Drautz-Moses, Scott A. Rice, Stephan Christoph Schuster, Zhao Zhi Boo, Bin Wu, Staffan Kjelleberg, Tim Tolker-Nielsen, Rajamani Lakshminarayanan, Roger W. Beuerman, Liang Yang, Michael Givskov
Institutions: Nanyang Technological University, Singapore Centre for Environmental Life Sciences Engineering, National University of Singapore, Singapore Eye Research Institute, National University Health System, Hong Kong Polytechnic University, BGI Group (China), BGI Genomics, Alfred-Wegener-Institut Helmholtz-Zentrum für Polar- und Meeresforschung, South China Agricultural University, ETH Zurich, Southern University of Science and Technology, Commonwealth Scientific and Industrial Research Organisation, Agriculture and Food, UNSW Sydney, University of Copenhagen, Duke-NUS Medical School, University of Tennessee Health Science Center
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W4289313577