In Vitro Acclimatization of Aseptically Cultured Plantlets to Humidity
Year: 1983
Type: article
Abstract: A method is described whereby relative humidity is reduced in the atmosphere of agar-solidified tissue cultures. Plantlets of chrysanthemum ( Chrysanthemum X morifolium Ramat.) cultured under low humidity exhibited a high mortality rate and those that survived were smaller and had few roots. Severe retardation of stomatal development occurred under reduced humidity and mature stomata had considerably narrower apertures than did plantlets grown at about 100% relative humidity. Plantlets of cauliflower ( Brassica oleracea L. Botrytis group) cultured under reduced humidity also exhibited reduced growth but, in addition, developed large quantities of surface wax and had lower rates of water loss. The results are discussed in relation to the potential uses of the technique for acclimatization of plantlets prior to transfer to soil.
Authors: K. Wardle, E.B. Dobbs, K.C. Short
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: hybrid
Estimated APC paid: $3,500
OpenAlex ID: W4289843530