Work

A protocol to use Drosophila melanogaster larvae to model human glioblastoma

Year: 2022

Type: article

Abstract: This protocol describes a genetic model system we developed for glioblastoma (GBM) in Drosophila melanogaster, which can be used to explore the pathogenic phenotypic effects of mutated genetic pathways and to identify potential therapeutic targets for tumors with these mutations. We present genetic schemes and experimental steps needed to create neoplastic glial brain tumors in larval Drosophila. We also provide steps to manipulate genes in this model and to perform brain fixation, immunostaining, and imaging of neoplastic larval brains. For complete details on the use and execution of this protocol, please refer to Read et al., (2009).

Source: STAR Protocols

DOI: 10.1016/j.xpro.2022.101609

Authors: Julia G. Saborio, Elizabeth E. Young, Alexander S. Chen, Renee D. Read

Institutions: Emory University, Winship Cancer Institute

Language: English

Topic: Glioma Diagnosis and Treatment

Subfield: Genetics

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,500