Work

Coordination of peptidoglycan synthesis and outer membrane constriction during Escherichia coli cell division

Year: 2015

Type: article

Abstract: To maintain cellular structure and integrity during division, Gram-negative bacteria must carefully coordinate constriction of a tripartite cell envelope of inner membrane, peptidoglycan (PG), and outer membrane (OM). It has remained enigmatic how this is accomplished. Here, we show that envelope machines facilitating septal PG synthesis (PBP1B-LpoB complex) and OM constriction (Tol system) are physically and functionally coordinated via YbgF, renamed CpoB (Coordinator of PG synthesis and OM constriction, associated with PBP1B). CpoB localizes to the septum concurrent with PBP1B-LpoB and Tol at the onset of constriction, interacts with both complexes, and regulates PBP1B activity in response to Tol energy state. This coordination links PG synthesis with OM invagination and imparts a unique mode of bifunctional PG synthase regulation by selectively modulating PBP1B cross-linking activity. Coordination of the PBP1B and Tol machines by CpoB contributes to effective PBP1B function in vivo and maintenance of cell envelope integrity during division.

Source: eLife

DOI: 10.7554/elife.07118

Authors: Andrew N Gray, Alexander JF Egan, Inge L van't Veer, Jolanda Verheul, Alexandre Colavin, Alexandra Koumoutsi, Jacob Biboy, A F Maarten Altelaar, Mirjam J Damen, Kerwyn Casey Huang, Jean-Pierre Simorre, Eefjan Breukink, Tanneke den Blaauwen, Athanasios Typas, Carol A Gross, Waldemar Vollmer

Institutions: University of California, San Francisco, Newcastle University, Utrecht University, University of Amsterdam, Stanford University, European Molecular Biology Laboratory, Stanford Medicine, Institut de Biologie Structurale, Université Grenoble Alpes, QB3

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,500

Funders: National Science Foundation, Alexander von Humboldt-Stiftung, Stanford University, Wellcome Trust, European Molecular Biology Laboratory, European Commission, Nederlandse Organisatie voor Wetenschappelijk Onderzoek, National Institutes of Health, National Institute of General Medical Sciences

Awards: Exploiting Gram-negative cell division targets in the test tube to obtain anti-microbial compounds, G4225002356, Bacterial Cell Wall Synthesis and Degradation., Systems Biology of Collective Cell Decisions, Gene Function and Pathway Analysis Using Systems Level Approaches in Prokaryotes, CAREER: Virtual Microbe: Biophysical Modeling of Morphogenesis, Bacterial Cell Wall Synthesis and Degradation., G8272570049

OpenAlex ID: W2169954205

FWCI: 14.19

Cites: 97

Cited by: 186

Related to: 10