m6A regulation of cortical and retinal neurogenesis is mediated by the redundant m6A readers YTHDFs
Year: 2022
Type: article
Abstract: m 6 A modification plays an important role in regulating mammalian neurogenesis. However, whether and how the major cytoplasmic m 6 A readers, YTHDF1, YTHDF2, and YTHDF3 mediate this process is still not clear. Here, we demonstrate that Ythdf1 and Ythdf2 double deletion but not individual knockout recapitulates the phenotype of Mettl14 knockout in cortex. In addition, we find that Mettl14 knockout in retina causes protracted proliferation of retinal progenitors, decreased numbers of retinal neurons, and disturbed laminar structure. This phenotype is only reproduced when Ythdf1 , Ythdf2 , and Ythdf3 are knocked out simultaneously in retina. Analysis of YTHDF target mRNAs in mouse cortex and retina reveals abundant overlapping mRNAs related to neurogenesis that are recognized and regulated by both YTHDF1 and YTHDF2. Together our results demonstrate that the functionally redundant YTHDFs mediate m 6 A regulation of cortical and retinal neurogenesis.
Source: iScience
Authors: Fugui Niu, Pengfei Che, Zhuoxuan Yang, Jian Zhang, Lixin Yang, Mengru Zhuang, Xijun Ou, Sheng-Jian Ji
Language: English
Topic: RNA modifications and cancer
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,000
OpenAlex ID: W4292170776