Work

An Evaluation of the Excised Root Tip Method for Determining the Resistance of Pisum sativum to Aphanomyces euteiches1

Year: 1971

Type: article

Abstract: Resistance in Pisum sativum to Aphanomyces euteiches was evaluated in the laboratory by the number of oospores formed in excised root tips inoculated with zoospores of the pathogen. Significantly lower mean numbers of oospores formed in root tips of 3 moderately resistant breeding lines than in those of 3 susceptible cultivars. Results were reproduceable if test procedures were adequately controlled. A sample size of 40 root tips gave 95% confidence in detecting a 40 oospore/root tip difference between means of resistant and susceptible genotypes. Lower mean numbers of oospores formed in excised root tips of genotypes which were resistant in greenhouse tests. Inoculum concentrations of 10 to 100 zoospores/plant caused severe disease in susceptible cv. New Era, while greater amounts of inoculum were needed to cause comparable disease in 2 resistant breeding lines.

Source: Journal of the American Society for Horticultural Science

DOI: 10.21273/jashs.96.5.616

Authors: R. H. Morrison, J. K. Johnson, T. H. King, D. Davis

Institution: University of Minnesota

Language: English

Topic: Plant Pathogens and Resistance

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: hybrid

Estimated APC paid: $3,500