Work

A microcarrier-based protocol for scalable generation and purification of human induced pluripotent stem cell-derived neurons and astrocytes

Year: 2022

Type: article

Abstract: Here, we describe a protocol for a microcarrier (MC)-based, large-scale generation and cryopreservation of human-induced pluripotent stem cell (hiPSC)-derived neurons and astrocytes. We also detail steps to isolate these populations with a high degree of purity. Finally, we describe how to cryopreserve these cell types while maintaining high levels of viability and preserving cellular function post-thaw. For complete details on the use and execution of this protocol, please refer to Brookhouser et al. (2021).

Source: STAR Protocols

DOI: 10.1016/j.xpro.2022.101632

Authors: Jacob Knittel, Gayathri Srinivasan, Carlye Frisch, Nicholas Brookhouser, Sreedevi Raman, Albert Essuman, David A. Brafman

Institutions: Arizona State University, University of Arizona College of Medicine- Phoenix

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,500

Funders: U.S. Department of Defense, International Foundation for Ethical Research, Arizona Biomedical Research Commission, National Institutes of Health, Office of the Secretary of Defense, National Institute on Aging

Awards: G2809824350, Using hiPSCs to investigate the protective mechanisms of the ApoEch mutation, G4721819199, Generation and characterization of isogenic hiPSC lines with various APOE genotypes, G6464158062, G7543709001, G8063383549, G9000134329

OpenAlex ID: W4292200786

FWCI: 0.069

Cites: 37

Cited by: 3

Related to: 10