Electrodeposited magnetic nanoporous membrane for high-yield and high-throughput immunocapture of extracellular vesicles and lipoproteins
Year: 2022
Type: article
Abstract: Superparamagnetic nanobeads offer several advantages over microbeads for immunocapture of nanocarriers (extracellular vesicles, lipoproteins, and viruses) in a bioassay: high-yield capture, reduction in incubation time, and higher capture capacity. However, nanobeads are difficult to “pull-down” because their superparamagnetic feature requires high nanoscale magnetic field gradients. Here, an electrodeposited track-etched membrane is shown to produce a unique superparamagnetic nano-edge ring with multiple edges around nanopores. With a uniform external magnetic field, the induced monopole and dipole of this nano edge junction combine to produce a 10× higher nanobead trapping force. A dense nanobead suspension can be filtered through the magnetic nanoporous membrane (MNM) at high throughput with a 99% bead capture rate. The yield of specific nanocarriers in heterogeneous media by nanobeads/MNM exceeds 80%. Reproducibility, low loss, and concentration-independent capture rates are also demonstrated. This MNM material hence expands the application of nanobead immunocapture to physiological samples.
Source: Communications Biology
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,490
Funders: U.S. Department of Health and Human Services, American Heart Association, Walther Cancer Foundation, China Scholarship Council, National Institutes of Health, National Heart, Lung, and Blood Institute, NIH Office of the Director, National Cancer Institute, National Institute of Diabetes and Digestive and Kidney Diseases, Office of Strategic Coordination, Common Fund
Awards: G1075853841, exRNA in colorectal carcinoma: biogenesis and function, High-Throughput Electrokinetic Fractionation and Analysis of Extracellular RNA Nano-Carriers, G4624152874, G4723475942, Regulation of High-Density Lipoprotein Function by Small RNA Modifications in Atherosclerosis-Associated Inflammation, G671486135, G7226719701, High-Throughput Electrokinetic Fractionation and Analysis of Extracellular RNA Nano-Carriers, G8763426593, Molecular and Cellular Basis for Digestive Diseases, Early to Late Events in Colorectal Neoplasia: Basic Biology to Clinical Implementation, Vanderbilt-Ingram Cancer Center SPORE in Gastrointestinal Cancer, HDL Function in Human Disease
OpenAlex ID: W4311667437