Work

Dissociation and reaggregation of Hydra vulgaris for studies of self-organization

Year: 2022

Type: article

Abstract: The remarkable regenerative abilities of the small cnidarian Hydra vulgaris include the capacity to reassemble itself after dissociation into individual cells. Here, we present a robust protocol for the dissociation and reaggregation of Hydra tissue that addresses many common challenges encountered during the preparation and execution of the dissociation, as well as the formation and care of regenerating cellular aggregates. Analysis of the process provides insight into the mechanisms of nervous system self-organization. For complete details on the use and execution of this protocol, please refer to Lovas and Yuste (2021).

Source: STAR Protocols

DOI: 10.1016/j.xpro.2022.101504

Authors: Jonathan R. Lovas, Rafael Yuste

Institutions: Marine Biological Laboratory, Columbia University

Language: English

Topic: Marine Invertebrate Physiology and Ecology

Subfield: Paleontology

Field: Earth and Planetary Sciences

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $1,500