Simple immunosensor for ultrasensitive electrochemical determination of biomarker of the bone metabolism in human serum
Year: 2022
Type: article
Abstract: Ultrasensitive and selective determination of biomarkers of the bone metabolism in serum is crucial for early screening, timely treatment, and monitoring of the curative effect of osteoporosis, which is a silent disease with serious health threats. Immunoassay with a simple sensing interface and ultrahigh sensitivity is highly desirable. Herein, a simple electrochemical immunosensor is demonstrated based on gold nanoparticles (AuNPs) electrodeposited on chitosan-reduced graphene oxide (CS-G) composite modified electrode, which can achieve sensitive determination of the important biomarker of bone metabolism, bone gamma-carboxyglutamate protein (BGP). To overcome the agglomeration of graphene and introduce a biocompatible matrix with functional amino groups, CS-G is prepared and modified on the supporting glassy carbon electrode (GCE). Then, AuNPs are electrodeposited on CS-G through their interaction between amine groups of CS. The immobilized AuNPs provide numerous binding sites to immobilize anti-BGP antibodies (AbBGP). The specific recognition between BGP and AbBGP results in a reduction in the mass transfer of the electrochemical probe (Fe(CN)63-/4-) in solution, leading to a reduced electrochemical signal. Based on this mechanism, fast and ultrasensitive electrochemical detection of BGP is achieved when the concentration of BGP ranges from 100 ag ml−1 to 10 μg mL−1 with a limit of detection (LOD) of 20 ag ml−1 (S/N = 3). The determination of BGP in human serum is also realized with high reliability.
Source: Frontiers in Chemistry
Authors: Qiang Chang, Jie Huang, Liming He, Fengna Xi
Language: English
Subfield: Electrical and Electronic Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,950
OpenAlex ID: W4293217248